Review of drebrin
Drebrin was discovered in 1985 by our group as a unique protein of which expression is transiently up-regulated in parallel with brain development. Since then, we purified drebrin protein, raised antibody against drebrin and cloned drebrin cDNA. Further we showed that drebrin is a unique actin-binding protein which is concentrated at dendritic spines. Please find the history of drebrin discovery in the reference list at early ages.
Drebrin plays a pivotal role in neuonal development (neurite growth and the growth cone shapes).
In paralell with further maturaion of neurons, drebrin isoform changes from enbryonic form (drebrin E) to adult neuron-specific form (drebrin A). The up-regulation of drebrin A facilitates the formation of dendritic spines.
In the mature neurons, drebrin A is specifically accumulated at dendritc spines. Interestingly the accumualtion of drebrin A depends on the synaptic activity, and LTP stimulation induce the transient exodus of dreb rin A from dendritic spines. As we see later, drebrin makes stable actin filaments; therefore the transient disappearance of drebrin from dendritic spines play a pivotal role for forming dynamic actin filaments, resulting in the morphological palstic change of dendritic spines.
In addition to the morphological synaptic plasticity, drebrin also plays a pivotal role of hoemostatic plasticity of the accumualtion of NMDA receptors at dendritic spines.
In 1996 we reported that massive disappearance of drebrin from neuropil occurs in the dimentia disorder such as Alzheimer's disease. This observation is consistent with the idea that persistent loss of drebrin from dendritic spines mayresult in the synaptic dysfunction. Furthermore this idea has been independently confirmed by various groups in the world using postmortem brain, animal model and cultured neurons. In addition it has been reportted that the cognitive function is quantitatively well correlated with the drebrin level in human brain.
Drebrin has ADF-H domain at N-terminal region, forming superfamily with cofilin and twinfilin. Drebrin is classified into two isoform groups: adult isoform (drebrin A, neuron-specific isoform) and embryonic isoform (drebrin E). Drebrin is reported to bound to various proteins, the most typical and important binding partner is an actin filament. Drebrin bounds to actin filaments and modify the helix pitch of actin polymers, resulting in the decrease of tread milling speed.
Drebrin plays a pivotal role in the formation of stable actin filaments in dendritic spines via decreasing the treadmill speed of actin filaments and increasing the helix pitch of actin filaments. The appearance of stable actin filaments in the core of dendritic spines is a marker of the mature synapse enabling synaptic plasticity. We are proposing that the accumulation of drebrin at dendritic spines is a good surrogate marker of the stable actin appearance in dendritic spines. This method is called DIBES (Drebrin-imaging-based evaluation of mature synapses), and are widely used for the screening of the effect of various chemical on neuronal synapses.
About Drebrin Antibody
Available antibodies against drebrin
|Name of antibody||species specificity||commercially available||raised animal|
|M2D6||none||only available from the Shirao laboratory||mouse monoclonal|
|M2H1||none||only available from the Shirao laboratory||mouse monoclonal|
|M2E6||chicken||only available from the Shirao laboratory||mouse monoclonal|
|M2A6||chicken||only available from the Shirao laboratory||mouse monoclonal|
|DAS2||mouse, rat, human||IBL||rabbit polycolnal|
MBL Tokyo Office: 03-3324-7331
MBL International Corporation 200 Dexter Avenue, Suite D, Watertown, MA 02172, USA
The contact address of IBL is
Immuno-Biological Laboratories, Inc. (IBL-America)
8201 Central Ave, NE Suite P,Minneapolis, MN 55432, USA
PHONE: +1 (763) 780-2955 FAX: +1 (763) 780-2988