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DIBES (Drebrin-imaging-based Evaluation of mature Synapses)

In this section, we introduce the easiest method by which the accumulation of drebrin at dendritic spines is reproducibly and quantitatively.

DIBES-EA2

As a marker of mature synapses, the appearance of slow tread-milling, stable actin filaments with 40 nm pitch is most appropriate. Because the stable actin inculde drebrin, the accumulation of drebrin  at dendritic spines is a good surrogate marker for the appearance of stable actin filaments. Using drebrin immunopositive clusters along dendrite r, the nuber of mature synapses is easily measured.

 

DIBES(only in Japanese)

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